The special issue “Spatial Information and participation of socio-ecological systems: experiences, tools and lessons learned for land-use planning”, focuses on a framework for information used in planning and participation processes for socio-ecological systems at different scales and contexts. The papers presented in the SI focus on three major questions: (1) How should we make use of spatial information for planning at different scales and contexts? (2) How should we share information among stakeholders and decision makers in land-use planning? (3) How can we communicate scientific knowledge to achieve effective decisions? The papers of this SI contribute original perspectives on how spatial information can be structured, used and communicated by/among different actors of land planning processes, creating more actionable scientific knowledge and effective related decision making.